Young farmers must overcome certain barriers when entering the sector. The aim is to evaluate the main obstacles with setting up of farming business and to assess whether the entry barriers of young farmers depend on the size of a farm. We use data from electronic survey among 510 young farmers in the Czech Republic. Average size of the farm was between 0 to 1000 ha (average 42.4 ha). The farmers were divided into categories: from 0 to 5 ha – small, over 5 ha to 50 ha – medium, and over 50 ha – large. Young farmers assessed the difficulties, which they had to overcome, when starting-up a farm on a scale from 1 (this was certainly a barrier) to 4 (it was not a huge barrier) and 0 (it is not my case). The average score for each category and barrier was calculated. The highest barrier was administrative burden and purchase of agricultural land. Young farmers did not perceive strategic planning and purchase of livestock as big problem. Test in contingency table revealed that almost all types of barriers statistically significantly depended on the size of the farm. For small and middle farms is the most difficult to start-up their businesses but purchase of agricultural land and obtaining knowledge and experience is difficult for all farms similarly.